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Chalcononaringenin 2′-O-glucoside (Ch2′G) was found to be the major pigment molecule in the petals of carnations bearing yellow flowers. The concentration of this pigment varied from 5.5 to 100.0% (relative value with amounts in the line ‘7154-03’ assumed to be 100%) in 31 carnation genotypes investigated. The transcription of both phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) gene s was active in the petals of both yellow carnation flowers and a cyanic control cultivar. The transcripts derived from the chalcone?flavanone isomerase (CHI) gene in the petals of yellow carnation flowers were below the level detectable by Northern blot analysis, but could be detected by RT-PCR. This is possible to produce subtle amounts of CHI protein translated from suspicious amounts of the mR     
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